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CAS NO.367-93-1
Product name:IPTG
Full name:Isopropyl-beta-D-thiogalactopyranoside
Content: 99% min by HPLC
Appearance: White Powder
Cas:367-93-1
MW:238.3012
MF:C9H18O5S
Isopropyl-β-D-thiogalactoside, often used in combination with X-Gal or Bluo-Gal, is used for blue and white screening of recombinant bacterial colonies. IPTG is not metabolized by bacteria and is a lactose analogue. Once it enters the cell, it will produce a long-lasting induction effect. Therefore, the induction efficiency of IPTG is high, and often only a small amount (1 mmol/L) is needed to achieve the ideal induction effect. Since IPTG is not metabolized, it specifically induces the expression of foreign proteins in the cell. Unaffected by cell metabolism, the induction effect remains stable.
Item |
Specification |
Description |
White Crystalline powder |
Solubility |
Soluble in water and methanol |
Identification by IR |
The IR spectrum of the sample should be concordant with the working standard |
pH 5% in water |
5.0-7.0 |
Melting Range |
110℃-114℃ |
Moisture Content by KF |
≤1.0 w/w |
1.4-Dioxane |
Should be absent |
SpecificOptical Rotatio |
-31.5±3.0° |
HPLC(on dry basis) |
≥99% |